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Vassart G. Constructs and mutagenesis Construction of the expression vector encoding PAX8 was described previously The in vitro synthesized PAX8 proteins were incubated with labeled oligonucleotides, separated via PAG, and visualized for details see Materials and Methods. Using a TTF1 expression construct as a control for nuclear localization, we found that the mutant PAX8 proteins localized correctly into the nucleus Fig.

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Corrias A. It is possible that this is altered in the case of RQ and, despite detectable protein—DNA interaction in the gel shift experiment the mutation may have an altered DNA-binding property. Due to the extreme variability of the thyroid phenotype, it is difficult to identify individuals harboring a PAX8 gene mutation. License type:. At the age of 7 years, her ultrasound was repeated and showed a small thyroid gland. Haaland and Reus combine to down Hoffenheim. Pirro MT. Lapi P.

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The extreme variability of the thyroid phenotype makes it difficult to identify individuals harboring PAX8 gene mutations. We performed in vitro studies to functionally characterize these mutations. However, RQ has no dominant negative effect on the WT protein in vitro.

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